Journal: Blood Research
Article Title: Hypermethylation-mediated silencing of RASD1 drives multiple myeloma pathogenesis
doi: 10.1007/s44313-026-00125-6
Figure Lengend Snippet: a MSP gel electrophoresis showing methylation (M) and unmethylation (U) status in representative samples. b RASD1 mRNA expression in U266 cells following DAC treatment. c Quantification of apoptosis rates (mean ± SD) in U266 cells (** P < 0.01). d Representative flow cytometry plots of apoptosis (Annexin V-YF647A/PI staining); Total apoptosis: 5.04% in the control group and 12.08% in the DAC-treated group
Article Snippet: The human MM cell line U266 was obtained from the American Type Culture Collection, and was maintained in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum.
Techniques: Nucleic Acid Electrophoresis, Methylation, Expressing, Flow Cytometry, Staining, Control